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A Schematic of DEX treatment during macrophage differentiation (day 0–6). B mRNA expression of the M1 markers CCR7 , CD80 , PTGS2 , and IL1B ( n = 3 [ CCR7 ], 4), and C ) M2 markers CD163 and MRC1 ( n = 3). D Schematic of DEX treatment during macrophage M1 polarization (day 6–8). E mRNA expression of M1 markers ( n = 3 [ PTGS2, IL1B ], 5 [ CCR7, CD80 ]), and F ) M2 markers ( n = 3, 4 [M2 0, M1 0]). H Schematic of DEX treatment of M1 polarized macrophages (days 8–10). I mRNA expression of M1 markers ( n = 3) and J M2 markers ( n = 3). M2 macrophages were not DEX-treated, and comparisons were to M1 polarized macrophages at 0 nM DEX in ( A )–( J ). K mRNA expression of the M2 markers CD163 and MRC1 , and NR3C1 (GR) in primary human peripheral blood monocyte-derived macrophages (hMDMs) after 2 days of vehicle or 100 nM DEX treatment. All mRNA expression was relative to untreated THP-1 cells and normalized to the housekeeping gene HPRT1 in B , C , E , F , I , J , and relative to untreated <t>CD14</t> + hPBM cells and normalized to HPRT1 in K. Bars represent mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001. Statistical significance was determined using one-way ANOVA followed by Dunnett’s multiple comparisons correction in B , C , E , F , I , J , and using Student’s unpaired t -test in ( K ).
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A Schematic of DEX treatment during macrophage differentiation (day 0–6). B mRNA expression of the M1 markers CCR7 , CD80 , PTGS2 , and IL1B ( n = 3 [ CCR7 ], 4), and C ) M2 markers CD163 and MRC1 ( n = 3). D Schematic of DEX treatment during macrophage M1 polarization (day 6–8). E mRNA expression of M1 markers ( n = 3 [ PTGS2, IL1B ], 5 [ CCR7, CD80 ]), and F ) M2 markers ( n = 3, 4 [M2 0, M1 0]). H Schematic of DEX treatment of M1 polarized macrophages (days 8–10). I mRNA expression of M1 markers ( n = 3) and J M2 markers ( n = 3). M2 macrophages were not DEX-treated, and comparisons were to M1 polarized macrophages at 0 nM DEX in ( A )–( J ). K mRNA expression of the M2 markers CD163 and MRC1 , and NR3C1 (GR) in primary human peripheral blood monocyte-derived macrophages (hMDMs) after 2 days of vehicle or 100 nM DEX treatment. All mRNA expression was relative to untreated THP-1 cells and normalized to the housekeeping gene HPRT1 in B , C , E , F , I , J , and relative to untreated <t>CD14</t> + hPBM cells and normalized to HPRT1 in K. Bars represent mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001. Statistical significance was determined using one-way ANOVA followed by Dunnett’s multiple comparisons correction in B , C , E , F , I , J , and using Student’s unpaired t -test in ( K ).
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primary peripheral blood cd14 monocytes pbmc human atcc product code  (ATCC)
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A Schematic of DEX treatment during macrophage differentiation (day 0–6). B mRNA expression of the M1 markers CCR7 , CD80 , PTGS2 , and IL1B ( n = 3 [ CCR7 ], 4), and C ) M2 markers CD163 and MRC1 ( n = 3). D Schematic of DEX treatment during macrophage M1 polarization (day 6–8). E mRNA expression of M1 markers ( n = 3 [ PTGS2, IL1B ], 5 [ CCR7, CD80 ]), and F ) M2 markers ( n = 3, 4 [M2 0, M1 0]). H Schematic of DEX treatment of M1 polarized macrophages (days 8–10). I mRNA expression of M1 markers ( n = 3) and J M2 markers ( n = 3). M2 macrophages were not DEX-treated, and comparisons were to M1 polarized macrophages at 0 nM DEX in ( A )–( J ). K mRNA expression of the M2 markers CD163 and MRC1 , and NR3C1 (GR) in primary human peripheral blood monocyte-derived macrophages (hMDMs) after 2 days of vehicle or 100 nM DEX treatment. All mRNA expression was relative to untreated THP-1 cells and normalized to the housekeeping gene HPRT1 in B , C , E , F , I , J , and relative to untreated <t>CD14</t> + hPBM cells and normalized to HPRT1 in K. Bars represent mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001. Statistical significance was determined using one-way ANOVA followed by Dunnett’s multiple comparisons correction in B , C , E , F , I , J , and using Student’s unpaired t -test in ( K ).
Primary Peripheral Blood Cd14 Monocytes Pbmc Human Atcc Product Code, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primary peripheral blood cd14 monocytes pbmc human atcc product code - by Bioz Stars, 2026-06
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A Schematic of DEX treatment during macrophage differentiation (day 0–6). B mRNA expression of the M1 markers CCR7 , CD80 , PTGS2 , and IL1B ( n = 3 [ CCR7 ], 4), and C ) M2 markers CD163 and MRC1 ( n = 3). D Schematic of DEX treatment during macrophage M1 polarization (day 6–8). E mRNA expression of M1 markers ( n = 3 [ PTGS2, IL1B ], 5 [ CCR7, CD80 ]), and F ) M2 markers ( n = 3, 4 [M2 0, M1 0]). H Schematic of DEX treatment of M1 polarized macrophages (days 8–10). I mRNA expression of M1 markers ( n = 3) and J M2 markers ( n = 3). M2 macrophages were not DEX-treated, and comparisons were to M1 polarized macrophages at 0 nM DEX in ( A )–( J ). K mRNA expression of the M2 markers CD163 and MRC1 , and NR3C1 (GR) in primary human peripheral blood monocyte-derived macrophages (hMDMs) after 2 days of vehicle or 100 nM DEX treatment. All mRNA expression was relative to untreated THP-1 cells and normalized to the housekeeping gene HPRT1 in B , C , E , F , I , J , and relative to untreated CD14 + hPBM cells and normalized to HPRT1 in K. Bars represent mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001. Statistical significance was determined using one-way ANOVA followed by Dunnett’s multiple comparisons correction in B , C , E , F , I , J , and using Student’s unpaired t -test in ( K ).

Journal: Cell Death & Disease

Article Title: Dexamethasone drives macrophage repolarization linked to increased triple-negative breast cancer aggressiveness

doi: 10.1038/s41419-025-08363-9

Figure Lengend Snippet: A Schematic of DEX treatment during macrophage differentiation (day 0–6). B mRNA expression of the M1 markers CCR7 , CD80 , PTGS2 , and IL1B ( n = 3 [ CCR7 ], 4), and C ) M2 markers CD163 and MRC1 ( n = 3). D Schematic of DEX treatment during macrophage M1 polarization (day 6–8). E mRNA expression of M1 markers ( n = 3 [ PTGS2, IL1B ], 5 [ CCR7, CD80 ]), and F ) M2 markers ( n = 3, 4 [M2 0, M1 0]). H Schematic of DEX treatment of M1 polarized macrophages (days 8–10). I mRNA expression of M1 markers ( n = 3) and J M2 markers ( n = 3). M2 macrophages were not DEX-treated, and comparisons were to M1 polarized macrophages at 0 nM DEX in ( A )–( J ). K mRNA expression of the M2 markers CD163 and MRC1 , and NR3C1 (GR) in primary human peripheral blood monocyte-derived macrophages (hMDMs) after 2 days of vehicle or 100 nM DEX treatment. All mRNA expression was relative to untreated THP-1 cells and normalized to the housekeeping gene HPRT1 in B , C , E , F , I , J , and relative to untreated CD14 + hPBM cells and normalized to HPRT1 in K. Bars represent mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001. Statistical significance was determined using one-way ANOVA followed by Dunnett’s multiple comparisons correction in B , C , E , F , I , J , and using Student’s unpaired t -test in ( K ).

Article Snippet: In addition, primary CD14 + human peripheral blood monocytes (hPBMs) were purchased from PromoCell (#C-14110) as a single-donor cell pellet stored in RNA later ® .

Techniques: Expressing, Derivative Assay